Journal: Virology

Article Title: Genetic analysis of the internal ribosome entry segment of bovine viral diarrhea virus.

doi: 10.1006/viro.1998.9425

Figure Lengend Snippet: FIG. 2. (A) Analysis of 59 end-dependent scanning and IRES-dependent reporter protein expression in vivo. CAT (solid bars) and LUC (striped bars) activity levels in lysates of CV-1 cells infected with vSP6 and transfected with DNA from each of the plasmids as indicated. (B) Effect of poliovirus infection on IRES-dependent translation. Catalytic activity of reporter proteins expressed in CV-1 cells infected with vSP6, transfected with pBi59BVDV (BVDV) or pBi59polio (polio), and superinfected with poliovirus strain Mahoney (cross-hatched bars) or mock-infected (bars with dotted pattern).

Article Snippet: Transfections were carried out at 37°C and 5% CO2 for 2 h. After transfection, the medium containing the DNA–lipid complexes was removed, and cells were supplemented with 1 ml of Dulbecco’s minimum essential medium and 10% fetal bovine serum for an additional 16 h. Poliovirus type I strain Mahoney (American Type Culture Collection) was propagated in Cos-7 cells.

Techniques: Expressing, In Vivo, Activity Assay, Infection, Transfection